研究業績

原著論文 (2020)

Involvement of sodium-coupled neutral amino acid transporters (system A) in L-proline transport in the rat retinal pericytes.
Zakoji N, Tajima K, Yoneyama D, Akanuma S, Kubo Y, Hosoya K.
Drug Metab Pharmacokinet. accepted for publication

Monocarboxylate Transporter 12 as a Guanidinoacetate Efflux Transporter in Renal Proximal Tubular Epithelial Cells.
Jomura R, Tanno Y, Akanuma S, Kubo Y, Tachikawa M, Hosoya K.
Biochim Biophys Acta Biomembr. 2020; 8:183434

Impact of Insoluble Separation Layer Mechanical Properties on Disintegration and Dissolution Kinetics of Multilayer Tablets.
Yokoyama R, Kimura G, Huwyler J, Hosoya K, Puchkov M.
Pharmaceutics. 2020; 12(6):495.

Predicting Method for the Human Plasma Concentration-Time Profile of a Monoclonal Antibody from the Half-life of Non-human Primates.
Nakamura G, Ozeki K, Nagayasu M, Nambu T, Nemoto T, Hosoya K.
Biol Pharm Bull. 2020; 43(3):823-830.

Determination of Intrinsic Creatine Transporter (Slc6a8) Activity and Creatine Transport Function of Leukocytes in Rats.
Taii A, Tachikawa M, Ohta Y, Hosoya K, Terasaki T.
Biol Pharm Bull. 2020; 43(3):474-479.

Contribution of Prostaglandin Transporter OATP2A1/SLCO2A1 to Placenta-to-Maternal Hormone Signaling and Labor Induction.
Inagaki M, Nishimura T, Nakanishi T, Shimada H, Noguchi S, Akanuma S, Tachikawa M, Hosoya K, Tamai I, Nakashima E, Tomi M.
iScience. 2020; 23(5):101098.

Polarized hemichannel opening of pannexin 1/connexin 43 contributes to dysregulation of transport function in blood-brain barrier endothelial cells.
Tachikawa M, Murakami K, Akaogi R, Akanuma SI, Terasaki T, Hosoya KI.
Neurochem Int. 2020; 132:104600.

Developmental changes in transporter and receptor protein expression levels at the rat blood-brain barrier based on quantitative targeted absolute proteomics.
Omori K, Tachikawa M, Hirose S, Taii A, Akanuma SI, Hosoya KI, Terasaki T.
Drug Metab Pharmacokinet. 2020; 35(1):117-123.

総説・著書等 (2020)

Foreword.
Ishida T, Kawakami S, Hosoya K.
Biol Pharm Bull. 2020; 43(4):575.

原著論文 (2019)

Inhibition of human microsomal PGE2 synthase-1 reduces seizure-induced increases of P-glycoprotein expression and activity at the blood-brain barrier.
Soldner ELB, Hartz AMS, Akanuma SI, Pekcec A, Doods H, Kryscio RJ, Hosoya KI, Bauer B.
FASEB J. 2019 Dec;33(12):13966-13981

Riboflavin transport mediated by riboflavin transporters (RFVTs/SLC52A) at the rat outer blood-retinal barrier.
Kubo Y, Miki S, Akanuma SI, Hosoya KI.
Drug Metab Pharmacokinet. 2019 Dec;34(6):380-386.

OCTN2-Mediated Acetyl-l-Carnitine Transport in Human Pulmonary Epithelial Cells In Vitro.
Salomon JJ, Gausterer JC, Selo MA, Hosoya KI, Huwer H, Schneider-Daum N, Lehr CM, Ehrhardt C.
Pharmaceutics. 2019 Aug 7;11(8). pii: E396.

Organic anion-transporting polypeptide 1a4-mediated heterogeneous distribution of sulforhodamine-101 in rat hepatic lobules.
Akanuma SI, Kida R, Tsuchiyama A, Tachikawa M, Kubo Y, Hosoya KI.
Drug Metab Pharmacokinet. 2019 Aug;34(4):239-246.

総説・著書等 (2019)

蛍光標識体を用いた血液網膜関門輸送機能の解明.
久保 義行.
ファルマシア.2019; 55(12): 1145-1149

In vivo analysis to study transport across the blood-retinal barrier.
Kubo Y, Akanuma S, Hosoya K.
In Blood-brain barrier (Barichello T Editor; Springer Protocols, Humana Press). 2019; p. 249-265

Techniques for evaluating efflux transport of radiolabeled drugs and compounds from the cerebrospinal fluid across the blood-cerebrospinal fluid barrier.
Akanuma S, Kubo Y, Hosoya K.
In Blood-brain barrier (Barichello T Editor; Springer Protocols, Humana Press). 2019; p. 231-248

原著論文 (2018)

Multiple Cellular Transport and Binding Processes of Unesterified Docosahexaenoic Acid in Outer Blood-Retinal Barrier Retinal Pigment Epithelial Cells.
Tachikawa M, Akanuma SI, Imai T, Okayasu S, Tomohiro T, Hatanaka Y, Hosoya KI.
BBiol Pharm Bull. 2018;41(9):1384-1392.

Investigation of receptor-mediated cyanocobalamin (vitamin B12) transport across the inner blood-retinal barrier using fluorescence-labeled cyanocobalamin.
Kinoshita Y, Nogami K, Jomura R, Akanuma SI, Abe H, Inouye M, Kubo Y, Hosoya KI.
Mol Pharm. 2018 Aug 6;15(8):3583-3594.

Recent advances in drug and nutrient transport across the blood-retinal barrier.
Kubo Y, Akanuma SI, Hosoya KI.
Expert Opin Drug Metab Toxicol. 2018 May;14(5):513-531.

Role of l-Type Amino Acid Transporter 1 at the Inner Blood-Retinal Barrier in the Blood-to-Retina Transport of Gabapentin.
Akanuma SI, Yamakoshi A, Sugouchi T, Kubo Y, Hartz AMS, Bauer B, Hosoya KI.
Mol Pharm. 2018 Jun 4;15(6):2327-2337.

Blood-to-Retina Transport of Fluorescence-Labeled Verapamil at the Blood-Retinal Barrier.
Kubo Y, Nakazawa A, Akanuma SI, Hosoya KI.
Pharm Res. 2018 Mar 12;35(5):93.

Gene expression of A6-like subgroup of ATP-binding cassette transporters in mouse brain parenchyma and microvessels.
Tachikawa M, Toki H, Watanabe M, Tomi M, Hosoya KI, Terasaki T.
Anat Sci Int. 2018 Mar 8.

Expression and function of connexin 43 protein in mouse and human retinal pigment epithelial cells as hemichannels and gap junction proteins.
Akanuma SI, Higashi H, Maruyama S, Murakami K, Tachikawa M, Kubo Y, Hosoya KI.
Exp Eye Res. 2018 Mar;168:128-137.

Developmental changes of l-arginine transport at the blood-brain barrier in rats.
Tachikawa M, Hirose S, Akanuma SI, Matsuyama R, Hosoya KI.
Microvasc Res. 2018 May;117:16-21.

Astrocytic γ-aminobutyric acid (GABA) transporters mediate guanidinoacetate transport in rat brain.
Tachikawa M, Yashiki A, Akanuma SI, Matsukawa H, Ide S, Minami M, Hosoya KI.
Neurochem Int. 2018 Feb;113:1-7.

原著論文 (2017)

Inner Blood-Retinal Barrier Dominantly Expresses Breast Cancer Resistance Protein: Comparative Quantitative Targeted Absolute Proteomics Study of CNS Barriers in Pig.
Zhang Z, Uchida Y, Hirano S, Ando D, Kubo Y, Auriola S, Akanuma SI, Hosoya KI, Urtti A, Terasaki T, Tachikawa M.
Mol Pharm. 2017 Nov 6;14(11):3729-3738.

Involvement of Carrier-Mediated Transport at the Blood-Cerebrospinal Fluid Barrier in Spermine Clearance from Rat Brain.
Akanuma SI, Shimada H, Kubo Y, Hosoya KI.
Biol Pharm Bull. 2017;40(9):1599-1603.

Co-localization of microsomal prostaglandin E synthase-1 with cyclooxygenase-1 in layer II of murine placental syncytiotrophoblasts.
Inagaki M, Nishimura T, Akanuma SI, Nakanishi T, Tachikawa M, Tamai I, Hosoya KI, Nakashima E, Tomi M.
Placenta. 2017 May;53:76-82.

Retina-to-Blood Transport of 1-Methyl-4-Phenylpyridinium Involves Carrier-Mediated Process at the Blood-Retinal Barrier.
Kubo Y, Yamamoto M, Matsunaga K, Usui T, Akanuma SI, Hosoya KI.
J Pharm Sci. 2017 Sep;106(9):2583-2591.

Current Progress Toward a Better Understanding of Drug Disposition Within the Lungs: Summary Proceedings of the First Workshop on Drug Transporters in the Lungs.
Ehrhardt C, Bäckman P, Couet W, Edwards C, Forbes B, Fridén M, Gumbleton M, Hosoya KI, Kato Y, Nakanishi T, Takano M, Terasaki T, Yumoto R.
J Pharm Sci. 2017 Sep;106(9):2234-2244.

Blood-to-retina transport of riboflavin via RFVTs at the inner blood-retinal barrier.
Kubo Y, Yahata S, Miki S, Akanuma SI, Hosoya KI.
Drug Metab Pharmacokinet. 2017 Feb;32(1):92-99.

原著論文 (2016)

Impact of SLC6A Transporters in Physiological Taurine Transport at the Blood-Retinal Barrier and in the Liver.
Kubo Y, Akanuma SI, Hosoya KI.
Biol Pharm Bull. 2016;39(12):1903-1911.
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Biol Pharm Bull表紙

Functional expression of nicotine influx transporter in A549 human alveolar epithelial cells.
Tega Y, Yuzurihara C, Kubo Y, Akanuma S, Ehrhardt C, Hosoya K.
Drug Metab Pharmacokinet. 2016 Feb;31(1):99-101.

原著論文 (2015)

Functional Characterization of Carrier-Mediated Transport of Pravastatin across the Blood-Retinal Barrier in Rats.
Fujii S, Setoguchi C, Kawazu K, Hosoya K.
Drug Metab Dispos. 2015 Dec;43(12):1956-9.

Impact of Cationic Amino Acid Transporter 1 on Blood-Retinal Barrier Transport of L-Ornithine.
Kubo Y, Obata A, Akanuma S, Hosoya K.
Invest Ophthalmol Vis Sci. 2015 Sep 1;56(10):5925-32.

Organic cation transporter function in different in vitro models of human lung epithelium.
Salomon JJ, Gausterer JC, Yahara T, Hosoya K, Huwer H, Hittinger M, Schneider-Daum N, Lehr CM, Ehrhardt C.
Eur J Pharm Sci. 2015 Dec 1;80:82-8.

Beta-2 Adrenergic Agonists Are Substrates and Inhibitors of Human Organic Cation Transporter 1.
Salomon JJ, Hagos Y, Petzke S, Kühne A, Gausterer JC, Hosoya K, Ehrhardt C.
Mol Pharm. 2015 Aug 3;12(8):2633-41.

Prostaglandin Transporter (PGT/SLCO2A1) Protects the Lung from Bleomycin-Induced Fibrosis.
Nakanishi T, Hasegawa Y, Mimura R, Wakayama T, Uetoko Y, Komori H, Akanuma SI, Hosoya KI, Tamai I.
PLoS One. 2015 Apr 29;10(4):e0123895.

Involvement of cationic amino Acid transporter 1 in L-arginine transport in rat retinal pericytes.
Zakoji N, Akanuma S, Tachikawa M, Hosoya K.
Biol Pharm Bull. 2015;38(2):257-62.

Contribution of pannexin 1 and connexin 43 hemichannels to extracellular calcium-dependent transport dynamics in human blood-brain barrier endothelial cells.
Kaneko Y, Tachikawa M, Akaogi R, Fujimoto K, Ishibashi M, Uchida Y, Couraud PO, Ohtsuki S, Hosoya K, Terasaki T.
J Pharmacol Exp Ther. 2015 Apr;353(1):192-200.

Involvement of the h+/organic cation antiporter in nicotine transport in rat liver.
Tega Y, Akanuma S, Kubo Y, Hosoya K.
Drug Metab Dispos. 2015 Jan;43(1):89-92.

原著論文 (2014)

Pharmacological significance of prostaglandin e2 and d2 transport at the brain barriers.
Tachikawa M, Hosoya K, Terasaki T.
Adv Pharmacol. 2014;71:337-60.

Involvement of carrier-mediated transport in the retinal uptake of clonidine at the inner blood-retinal barrier.
Kubo Y, Tsuchiyama A, Shimizu Y, Akanuma S, Hosoya K.
Mol Pharm. 2014 Oct 6;11(10):3747-53.

Transporter-mediated Prostaglandin E2 Elimination across the Rat Blood-brain Barrier and Its Attenuation by the Activation of N-methyl-D-aspartate Receptors.
Akanuma S, Higuchi T, Higashi H, Ozeki G, Tachikawa M, Kubo Y, Hosoya K.
Drug Metab Pharmacokinet. 2014 Oct 25;29(5):387-93.

Distribution of microsomal prostaglandin E synthase-1 in the mouse brain.
Eskilsson A, Tachikawa M, Hosoya K, Blomqvist A.
J Comp Neurol. 2014 Dec 1;522(14):3229-44.

Involvement of the carrier-mediated process in the retina-to-blood transport of spermine at the inner blood-retinal barrier.
Kubo Y, Tomise A, Tsuchiyama A, Akanuma SI, Hosoya KI.
Exp Eye Res. 2014 May 14;124:17-23.

Amino Acid Residues Involved in the Substrate Specificity of TauT/SLC6A6 for Taurine and γ-Aminobutyric Acid.
Yahara T, Tachikawa M, Akanuma S, Kubo Y, Hosoya K.
Biol Pharm Bull. 2014;37(5):817-25.

Pharmacokinetics of guanidinosuccinic acid in rat blood and cerebrospinal fluid.
Kasai Y, Akanuma S, Kubo Y, Tachikawa M, Hosoya K.
Drug Metab Pharmacokinet. 2014;29(1):97-100.

原著論文 (2013)

Quantitative targeted absolute proteomic analysis of transporters, receptors and junction proteins for validation of human cerebral microvascular endothelial cell line hCMEC/D3 as a human blood-brain barrier model.
Ohtsuki S, Ikeda C, Uchida Y, Sakamoto Y, Miller F, Glacial F, Decleves X, Scherrmann JM, Couraud PO, Kubo Y, Tachikawa M, Terasaki T.
Mol Pharm. 2013;10:289-96.

Localization of organic anion transporting polypeptide (Oatp) 1a4 and Oatp1c1 at the rat blood-retinal barrier.
Akanuma S, Hirose S, Tachikawa M, Hosoya K.
Fluids Barriers CNS. 2013 Oct 2;10(1):29.

Propranolol transport across the inner blood-retinal barrier: potential involvement of a novel organic cation transporter.
Kubo Y, Shimizu Y, Kusagawa Y, Akanuma S, Hosoya K.
J Pharm Sci. 2013 Sep;102(9):3332-42.

Na+-independent nucleoside transporters regulate adenosine and hypoxanthine levels in Müller cells and the inner blood-retinal barrier.
Akanuma S, Soutome T, Hisada E, Tachikawa M, Kubo Y, Hosoya K.
Invest Ophthalmol Vis Sci. 2013 Feb 27;54(2):1469-77.

Involvement of a novel organic cation transporter in verapamil transport across the inner blood-retinal barrier.
Kubo Y, Kusagawa Y, Tachikawa M, Akanuma S, Hosoya K.
Pharm Res. 2013 Mar;30(3):847-56.

著書・総説

久保義行,赤沼伸乙,細谷健一: カチオン性薬物輸送に見る血液網膜関門と血液脳関門の類似と相違.細胞工学.2013;32:950-4.

立川正憲,赤沼伸乙,寺崎哲也: 脳関門プロスタグランジン輸送の分子病態生理.細胞工学.2013;32:946-9.

原著論文 (2012)

The inner blood-retinal barrier: molecular structure and transport biology.
Hosoya K, Tachikawa M.
Adv Exp Med Biol. 2012;763:85-104.

Role of the blood-cerebrospinal fluid barrier transporter as a cerebral clearance system for prostaglandin E₂ produced in the brain.
Tachikawa M, Ozeki G, Higuchi T, Akanuma S, Tsuji K, Hosoya K.
J Neurochem. 2012 Dec;123(5):750-60.

A clearance system for prostaglandin D2, a sleep-promoting factor, in cerebrospinal fluid: role of the blood-cerebrospinal barrier transporters.
Tachikawa M, Tsuji K, Yokoyama R, Higuchi T, Ozeki G, Yashiki A, Akanuma S, Hayashi K, Nishiura A, Hosoya K.
J Pharmacol Exp Ther. 2012 Dec;343(3):608-16.
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The Journal of pharmacology and experimental therapeutics表紙
JPET表紙

Quantitative prediction of human intestinal glucuronidation effects on intestinal availability of UDP-glucuronosyltransferase substrates using in vitro data.
Nakamori F, Naritomi Y, Hosoya K, Moriguchi H, Tetsuka K, Furukawa T, Kadono K, Yamano K, Terashita S, Teramura T.
Drug Metab Dispos. 2012 Sep;40(9):1771-7.

Involvement of γ-aminobutyric acid transporter 2 in the hepatic uptake of taurine in rats.
Ikeda S, Tachikawa M, Akanuma S, Fujinawa J, Hosoya K.
Am J Physiol Gastrointest Liver Physiol. 2012 Aug 1;303(3):G291-7.

Application of membrane permeability evaluated in in vitro analyses to estimate blood-retinal barrier permeability.
Kubo Y, Fukui E, Akanuma S, Tachikawa M, Hosoya K.
J Pharm Sci. 2012 Jul;101(7):2596-605.

Function and regulation of taurine transport in Müller cells under osmotic stress.
Ando D, Kubo Y, Akanuma S, Yoneyama D, Tachikawa M, Hosoya K.
Neurochem Int. 2012 May;60(6):597-604.

γ-Aminobutyric acid transporter 2 mediates the hepatic uptake of guanidinoacetate, the creatine biosynthetic precursor, in rats.
Tachikawa M, Ikeda S, Fujinawa J, Hirose S, Akanuma S, Hosoya K.
PLoS One. 2012;7(2):e32557.

Up-regulation of L-type amino acid transporter 1 (LAT1) in cultured rat retinal capillary endothelial cells in response to glucose deprivation.
Matsuyama R, Tomi M, Akanuma S, Tabuchi A, Kubo Y, Tachikawa M, Hosoya K.
Drug Metab Pharmacokinet. 2012;27(3):317-24.

著書・総説

Kubo Y., and Hosoya K. : Inner blood-retinal barrier transporters: relevance to diabetic retinopathy, In Diabetic Retinopathy, by Ola M.S.(Ed), 91-108, InTech, Croatia, 2012.

Tachikawa M., Hosoya K., Smith S.B., Martin P.M., and Ganapathy V. : Transport of drugs across the inner and outer blood-retinal barriers: relevance of transporters in the retinal blood vessel endothelium and the retinal pigment epithelium, In Advances in Ocular Drug Delivery, by Mitra A.K.(Ed), 1-31, Research Signpost Publication, Kerala, 2012.

Hosoya K., and Tachikawa M. : The inner blood-retinal barrier: molecular structure and transport biology, In Biology and Regulation of Blood-Tissue Barriers, by Cheng C.Y.(Ed), 85-104, Springer, New York, 2012.

細谷健一 : 薬物の分布. 「パートナー薬剤学 改訂第2版」寺田勝英, 伊藤智夫編, 162-177, 南江堂, 東京, 2012.

細谷健一 : 血液網膜関門輸送系の分子機構と薬物輸送. 眼薬理, 26: 50-53, 2012.

久保義行, 赤沼伸乙, 細谷健一 : 血液網膜関門の機能特性を利用したドラッグデリバリー. Drug Delivery Syst., 27: 361-369, 2012.

原著論文 (2011)

Molecular-weight-dependent, anionic-substrate-preferential transport of β-lactam antibiotics via multidrug resistance-associated protein 4.
Akanuma S, Uchida Y, Ohtsuki S, Kamiie J, Tachikawa M, Terasaki T, Hosoya K.
Drug Metab Pharmacokinet. 2011;26(6):602-11.

Retinal transfer of nicotinate by H+ -monocarboxylate transporter at the inner blood-retinal barrier.
Tachikawa M, Murakami K, Martin PM, Hosoya K, Ganapathy V.
Microvasc Res. 2011 Nov;82(3):385-90.

Influence of insulin on glutamine synthetase in the Müller glial cells of retina.
Ola MS, Hosoya K, LaNoue KF.
Metab Brain Dis. 2011 Sep;26(3):195-202.

Transport systems of serine at the brain barriers and in brain parenchymal cells.
Kasai Y, Tachikawa M, Hirose S, Akanuma S, Hosoya K.
J Neurochem. 2011 Jul;118(2):304-13.

Inner blood-retinal barrier mediates l-isomer-predominant transport of serine.
Tachikawa M, Okamoto M, Hirose S, Yoneyama D, Akanuma S, Terasaki T, Hosoya K.
J Pharm Sci. 2011 Sep;100(9):3892-903.

Amyloid-β peptide(1-40) elimination from cerebrospinal fluid involves low-density lipoprotein receptor-related protein 1 at the blood-cerebrospinal fluid barrier.
Fujiyoshi M, Tachikawa M, Ohtsuki S, Ito S, Uchida Y, Akanuma S, Kamiie J, Hashimoto T, Hosoya K, Iwatsubo T, Terasaki T.
J Neurochem. 2011 Aug;118(3):407-15.

Reactive oxygen species downregulate glucose transport system in retinal endothelial cells.
Fernandes R, Hosoya K, Pereira P.
Am J Physiol Cell Physiol. 2011 Apr;300(4):C927-36.

Response of rat retinal capillary pericytes and endothelial cells to glucose.
Makita J, Hosoya K, Zhang P, Kador PF.
J Ocul Pharmacol Ther. 2011 Feb;27(1):7-15.

著書・総説

Tachikawa M., Ganapathy V., and Hosoya K. : Systemic route for retinal drug delivery: role of the blood-retinal barrier, In Drug Product Development for the Back of the Eye, by Kompella U.B. and Edelhauser H.F.(Ed.), 85-109, Springer, New York, 2011.

大塚 誠,細谷健一:製剤の種類.「わかりやすい物理薬剤学」第5版,辻 彰,河島 進編, 187-191, 廣川書店, 東京, 2011.

細谷健一,大塚 誠:製剤の種類.「わかりやすい物理薬剤学」第5版,辻 彰,河島 進編, 192-210, 廣川書店, 東京, 2011.

寺崎哲也,大槻純男,久保義行,内田康雄,大峰 健:定量的トランスポーター解析のための質量分析による膜タンパク質絶対定量法.「トランスポートソームの世界」金井好克,竹島 浩,森泰生,久保義弘編, 405-410, 京都廣川書店, 東京, 2011.

Tachikawa M., and Hosoya K. : Transport characteristics of guanidino compounds at the blood-brain barrier and blood-cerebrospinal fluid barrier: relevance to neural disorders. Fluids Barriers CNS, 8:13, 2011.

Hosoya K., and Tachikawa M. : Roles of organic anion/cation transporters at the blood-brain and blood-cerebrospinal fluid barriers involving uremic toxins. Clin. Exp. Nephrol., 15: 478-485, 2011.

Hosoya K., Tomi M., and Tachikawa M. : Strategies for therapy of retinal diseases using systemic drug delivery: relevance of transporters at the blood-retinal barrier. Expert. Opin. Drug Deliv., 8: 1571-1587, 2011.

Ohtsuki S., Uchida Y., Kubo Y., and Terasaki T. : Quantitative targeted absolute proteomics-based ADME research as a new path to drug discovery and development: methodology, advantages, strategy, and prospects. J. Pharm. Sci., 100: 3547-3559, 2011.