title.jpg

main.jpg
research.jpg
members.jpg
publications.jpg
photos.jpg
history.jpg
access.jpg
english.jpg






連絡先
〒930-0194
富山市杉谷2630
富山大学学術研究部薬学･和漢系
生体界面化学研究室
　　TEL: 076-434-7565
　　FAX: 076-434-7568

mark.jpg
mark2.jpg

 論　文

  • Synergistic Effects of Hydrophilic Residues in the Transmembrane Region on Lipid Scrambling Activity of Dimeric Helices.
    H. Nakao, T. Tsujii, H. Saito, K. Ikeda, M. Nakano
    Colloid Surf. B: Biointerfaces 251, 114612 (2025).
    [doi.org/10.1016/j.colsurfb.2025.114612]

  • Thermodynamics of Oligomerization and Helix-to-Sheet Structural Transition of Amyloid β-Protein on Anionic Phospholipid Vesicles.
    K. Ikeda, Y. Sugiura, H. Nakao, M. Nakano
    Biophys. Chem. 310, 107248 (2024).
    [doi.org/10.1016/j.bpc.2024.107248]

  • Impact of Transmembrane Peptides on Individual Lipid Motions and Collective Dynamics of Lipid Bilayers.
    H. Nakao, M. Nagao, T. Yamada, K. Imamura, K. Nozaki, K. Ikeda, M. Nakano
    Colloid Surf. B: Biointerfaces 228, 113396 (2023).
    [doi.org/10.1016/j.colsurfb.2023.113396]
  • Fine-Tuning and Enhancement of pH-Dependent Membrane Permeation of Cyclic Peptides by Utilizing Noncanonical Amino Acids with Extended Side Chains.
    M. Matsuda, K. Ikeda, T. Kameda, H. Nakao, M. Nakano
    J. Med. Chem. 66(10), 7054-7062 (2023).
    [doi.org/10.1021/acs.jmedchem.3c00628]
  • Nanometer-Scale Proximity of Bilayers Facilitates Intermembrane Lipid Transfer.
    A. Miyajima, H. Nakao, K. Ikeda, M. Nakano
    J. Phys. Chem. Lett. 14(18), 4172-4178 (2023).
    [doi.org/10.1021/acs.jpclett.3c00154]
  • Energy Diagram of Phospholipid Transfer from Membranes with Different Curvature Revealed by Time-Resolved Neutron Scattering.
    M. Nakano, H. Nakao
    Neutron News 34(2), 10-12 (2023).
    [doi.org/10.1080/10448632.2023.2191573]
  • Rational Design of Phase Separating Peptides Based on Phase Separating Protein Sequence of p53.
    K. Kamagata, A. Hando, M. Ariefai, N. Iwaki, S. Kanbayashi, R. Koike, K. Ikeda
    Sci. Rep. 13, 5648 (2023).
    [doi.org/10.1038/s41598-023-32632-2]
  • Amphipathic Peptide-Phospholipid Nanofibers: Kinetics of Fiber Formation and Molecular Transfer between Assemblies.
    C. Shimizu, K. Ikeda, H. Nakao, M. Nakano
    Biophys. Chem. 296, 106985 (2023).
    [doi.org/10.1016/j.bpc.2023.106985]

  • Rational Peptide Design for Regulating Liquid-Liquid Phase Separation on the Basis of Residue–Residue Contact Energy.
    K. Kamagata, M. Ariefai, H. Takahashi, A. Hando, D. R. G. Subekti, K. Ikeda, A. Hirano, T. Kameda
    Sci. Rep. 12, 13718 (2022).
    [doi.org/10.1038/s41598-022-17829-1]
  • Flip-Flop Promotion Mechanisms by Model Transmembrane Peptides. (Review)
    H. Nakao, M. Nakano
     Chem. Pharm. Bull. 70(8), 519-523 (2022).
    [doi.org/10.1248/cpb.c22-00133]
  • Energetic and Structural Insights into Phospholipid Transfer from Membranes with Different Curvatures by Time-Resolved Neutron Scattering.
    M. Nakano, H. Nakao, S. Yoshida, M. Fukuda, M. Imai, K. Ikeda
    J. Phys. Chem. Lett. 13(26), 6024-6030 (2022).
    [doi.org/10.1021/acs.jpclett.2c01080]
  • Kinetic Mechanism of Amyloid-β-(16–22) Peptide Fibrillation.
    M. Yamazaki, K. Ikeda, T. Kameda, H. Nakao, M. Nakano
    J. Phys. Chem. Lett. 13(26), 6031-6036 (2022).
    [doi.org/10.1021/acs.jpclett.2c01065]
  • Improvement of Thermal Stability of Amphipathic Peptide–Phospholipid Nanodiscs via Lateral Association of α-Helices by Disulfide Cross-Linking.
    C. Anada, K. Ikeda, H. Nakao, M. Nakano
    Langmuir 38(22), 6977–6983 (2022).
    [doi.org/10.1021/acs.langmuir.2c00533]

  • Temperature- and Composition-Dependent Conformational Transitions of Amphipathic Peptide-Phospholipid Nanodiscs.
    C. Anada, K. Ikeda, A. Egawa, T. Fujiwara, H. Nakao, M. Nakano
    J. Colloid Interface Sci. 588, 522-530 (2021).
    [doi.org/10.1016/j.jcis.2020.12.090]
  • Characterization of Design Grammar of Peptides for Regulating Liquid Droplets and Aggregates of FUS.
    K. Kamagata, R. Chiba, I. Kawahata, N. Iwaki, S. Kanbayashi, K. Maeda, H. Takahashi, A. Hirano, K. Fukunaga, K. Ikeda, T. Kameda
    Sci. Rep. 11, 6643 (2021).
    [doi.org/10.1038/s41598-021-86098-1]
  • Development of Membrane-Insertable Lipid Scrambling Peptides: A Time-Resolved Small-Angle Neutron Scattering Study.
    H. Nakao, Y. Kimura, A. Sakai, K. Ikeda, M. Nakano
    Structural Dynamics 8, 024301 (2021).
    [doi.org/10.1063/4.0000045]
  • Amphipathic Peptide-Phospholipid Nanofibers: Phospholipid Specificity and Dependence on Concentration and Temperature.
    K. Ikeda, A. Horiuchi, M. Yoshino, C. Shimizu, H. Nakao, M. Nakano
    Langmuir 37(2), 713-721 (2021).
    [doi.org/10.1021/acs.langmuir.0c02819]
  • Biophysical Parameters of the Sec14 Phospholipid Exchange Cycle – Effect of Lipid Packing in Membranes.
    T. Sugiura, H. Nakao, K. Ikeda, D. Khan, A. H. Nile, V. A. Bankaitis, M. Nakano
    Biochim. Biophys. Acta - Biomembranes 1863(1), 183450 (2021).
    [doi.org/10.1016/j.bbamem.2020.183450]

  • Urea-Assisted Reconstitution of Discoidal High-Density Lipoprotein.
    R. Fukuda, M. Saito, S. Shibukawa, A. Sumino, M. Nakano, T. Murakami
    Biochemistry 59(15), 1455-1464 (2020).
    [doi.org/10.1021/acs.biochem.0c00075]
  • Structural Feature of Lipid Scrambling Model Transmembrane Peptides: Same-Side Positioning of Hydrophilic Residues and Their Deeper Position.
    H. Nakao, Y. Sugimoto, K. Ikeda, H. Saito, M. Nakano 
    J. Phys. Chem. Lett. 11(5), 1662-1667 (2020).
    [doi.org/10.1021/acs.jpclett.0c00175]
  • Pro-Inflammatory Macrophages Coupled with Glycolysis Remodel Adipose Vasculature by Producing Platelet-Derived Growth Factor-B in Obesity.
    Y. Onogi, T. Wada, A. Okekawa, T. Matsuzawa, E. Watanabe, K. Ikeda, M. Nakano, M. Kitada, D. Koya, H. Tsuneki, T. Sasaoka
    Sci. Rep. 10, 670 (2020).
    [doi.org/10.1038/s41598-019-57368-w]

  • Novel Cubosome System Resistant to Lipid Removal by Serum Albumin.
    M. Uyama, T. Handa, M. Nakano
    Chem. Pharm. Bull. 67(10), 1099-1103 (2019).
    [doi.org/10.1248/cpb.c19-00370]
  • Evaluation of Interbilayer and Transbilayer Transfer Dynamics of Phospholipids Using Time-Resolved Small-Angle Neutron Scattering. (Review)
    M. Nakano
    Chem. Pharm. Bull. 67(4), 316-320 (2019).
    [dx.doi.org/10.1248/cpb.c18-00942]
  • Biophysical Parameters of the Sec14 Phospholipid Exchange Cycle.
    T. Sugiura, C. Takahashi, Y. Chuma, M. Fukuda, M. Yamada, U. Yoshida, H. Nakao, K. Ikeda, D. Khan, A. H. Nile, V. A. Bankaitis, M. Nakano
    Biophys. J. 116(1), 92-103 (2019).
    [dx.doi.org/10.1016/j.bpj.2018.11.3131]
  • Toxic Amyloid Tape: A Novel Mixed Antiparallel/Parallel β-Sheet Structure Formed by Amyloid β-Protein on GM1 Clusters.
    Y. Okada, K. Okubo, K. Ikeda, Y. Yano, M. Hoshino, Y. Hayashi, Y. Kiso, H. Itoh-Watanabe, A. Naito, K. Matsuzaki
    ACS Chem. Neurosci. 10(1), 563-572 (2019).
    [dx.doi.org/10.1021/acschemneuro.8b00424]

  • Discovery of a Sugar-Based Nanoparticle Universally Existing in Boiling Herbal Water Extracts and their Immunostimulant Effect.
    H. Iitsuka, K. Koizumi, A. Inujima, M. Suzaki, Y. Mizuno, Y. Takeshita, T. Eto, Y. Otsuka, R. Shimada, M. Liu, K. Ikeda, M. Nakano, R. Suzuki, K. Maruyama, Y. Zhou, H. Sakurai, N. Shibahara
    Biochem. Biophys. Rep. 16, 62-68 (2018).
    [dx.doi.org/10.1016/j.bbrep.2018.08.004]
  • Veratridine Binding to a Transmembrane Helix of Sodium Channel Nav1.4 Determined by Solid-State NMR.
    A. Niitsu, A. Egawa, K. Ikeda, K. Tachibana, T. Fujiwara
    Bioorg. Med. Chem. 26(21), 5644-5653 (2018).
    [dx.doi.org/10.1016/j.bmc.2018.10.012]
  • Effects of Hydrophilic Residues and Hydrophobic Length on Flip-Flop Promotion by Transmembrane Peptides.
    H. Nakao, C. Hayashi, K. Ikeda, H. Saito, H. Nagao, M. Nakano
    J. Phys. Chem. B 122(15), 4318-4324 (2018).
    [dx.doi.org/10.1021/acs.jpcb.8b00298]
  • Characterization of Human ATP-Binding Cassette Protein Subfamily D Reconstituted into Proteoliposomes.
    T. Okamoto, K. Kawaguchi, S. Watanabe, R. Agustina, T. Ikejima, K. Ikeda, M. Nakano, M. Morita, T. Imanaka
    Biochem. Biophys. Res. Commun. 496(4), 1122-1127 (2018).
    [dx.doi.org/10.1016/j.bbrc.2018.01.153]
  • Effect of Dialkyl Ammonium Cationic Surfactants on the Microfluidity of Membranes Containing Raft Domains.
    M. Uyama, K. Inoue, K. Kinoshita, R. Miyahara, H. Yokoyama, M.Nakano
    J. Oleo Sci. 67(1), 67-75 (2018).
    [dx.doi.org/10.5650/jos.ess17124]
  • Formation of Asymmetric Vesicles via Phospholipase D-Mediated Transphosphatidylation.
    R. Takaoka, H. Kurosaki, H. Nakao, K. Ikeda, M. Nakano
    Biochim. Biophys. Acta - Biomembranes 1860(2), 245-249 (2018).
    [dx.doi.org/10.1016/j.bbamem.2017.10.011]

  • Nanodisc-to-Nanofiber Transition of Noncovalent Peptide–Phospholipid Assemblies.
    K. Ikeda, A. Horiuchi, A. Egawa, H. Tamaki, T. Fujiwara, M.Nakano
    ACS Omega 2(6), 2935-2944 (2017).
    [dx.doi.org/10.1021/acsomega.7b00424]

  • Kinetic Analysis of the Methyl-β-Cyclodextrin-Mediated Intervesicular Transfer of Pyrene-Labeled Phospholipids.
    T. Sugiura, K. Ikeda, M. Nakano
    Langmuir 32(51), 13697-13705 (2016).
    [dx.doi.org/10.1021/acs.langmuir.6b03515]
  • Energetics of the Mixing of Phospholipids in Bilayers Determined Using Vesicle Solubilization.
    K. Ikeda, M. Nakano
    Langmuir 32(49), 13270-13275 (2016).
    [dx.doi.org/10.1021/acs.langmuir.6b03333]
  • Formation of Size-Controlled, Denaturation-Resistant Lipid Nanodiscs by an Amphiphilic Self-Polymerizing Peptide.
    H. Kondo, K. Ikeda, M. Nakano
    Colloid Surf. B: Biointerfaces 146, 423-430 (2016).
    [dx.doi.org/10.1016/j.colsurfb.2016.06.040]
  • Membrane-Spanning Sequences in Endoplasmic Reticulum Proteins Promote Phospholipid Flip-flop.
    H. Nakao, K. Ikeda, Y. Ishihama, M. Nakano
    Biophys. J. 110(12), 2689-2697 (2016).
    [dx.doi.org/10.1016/j.bpj.2016.05.023]

  • Preparation and Characterization of Reconstituted Lipid–Synthetic Polymer Discoidal Particles.
    M. Tanaka, A. Hosotani, Y. Tachibana, M. Nakano, K. Iwasaki, T.Kawakami, T. Mukai
    Langmuir 31, 12719-12726 (2015).
    [dx.doi.org/10.1021/acs.langmuir.5b03438]
  • High Membrane Curvatures Enhance Binding, Conformational Changes, and Fibrillation of Amyloid-β on Lipid Bilayer Surfaces.
    Y. Sugiura, K. Ikeda, M. Nakano
    Langmuir 31(46), 11549-11557 (2015).
    [dx.doi.org/10.1021/acs.langmuir.5b03332]
  • Kinetic and Thermodynamic Analysis of Cholesterol Transfer between Phospholipid Vesicles and Nanodiscs.
    N. Matsuzaki, T. Handa, M. Nakano
    J. Phys. Chem. B 119(30), 9764-9771 (2015).
    [dx.doi.org/10.1021/acs.jpcb.5b03682]
  • Chylomicron Remnant Model Emulsions Induce Intracellular Cholesterol Accumulation and Cell Death Due to Lysosomal Destabilization.
    K. Wakita, S. Morita, N. Okamoto, E. Takata, T. Handa, M. Nakano
    Biochim. Biophys. Acta - Molecular and Cell Biology of Lipids 1851(5), 598-604 (2015).
    [dx.doi.org/10.1016/j.bbalip.2015.01.015]
  • Self-Reproduction of Nanoparticles through Synergistic Self-Assembly.
    K. Ikeda, M. Nakano
    Langmuir 31(1), 17-21 (2015).
    [dx.doi.org/10.1021/la503491p]
  • pH-Dependent Promotion of Phospholipid Flip-Flop by the KcsA Potassium Channel.
    H. Nakao, K. Ikeda, M. Iwamoto, H. Shimizu, S. Oiki, Y. Ishihama, M.Nakano
    Biochim. Biophys. Acta - Biomembranes 1848(1), 145-150 (2015).
    [dx.doi.org/10.1016/j.bbamem.2014.10.001]

  • Effect of Phospholipid Composition on Discoidal HDL Formation.
    M. Miyazaki, Y. Tajima, Y. Ishihama, T. Handa, M. Nakano
    Biochim. Biophys. Acta - Biomembranes 1828(5), 1340-1346 (2013).
    [dx.doi.org/10.1016/j.bbamem.2013.01.012]
  • Control of Phospholipid Flip-Flop by Transmembrane Peptides.
    M. Kaihara, H. Nakao, H. Yokoyama, H. Endo, Y. Ishihama, T. Handa, M.Nakano
    Chem. Phys. 419, 78-83 (2013).
    [dx.doi.org/10.1016/j.chemphys.2012.12.041]
  • Effects of Lipid Membrane Curvature on Lipid Packing State Evaluated by Isothermal Titration Calorimetry.
    H. Yokoyama, K. Ikeda, M. Wakabayashi, Y. Ishihama, M. Nakano
    Langmuir 29(3), 857-860 (2013).
    [dx.doi.org/10.1021/la304532k]
  • Secondary Structural Analysis of Proteins Based on 13C Chemical Shift Assignments in Unresolved Solid-State NMR Spectra Enhanced by Fragmented Structure Database.
    K. Ikeda, A. Egawa, T. Fujiwara
    J. Biomol. NMR 55, 189-200 (2013).
    [dx.doi.org/10.1007/s10858-012-9701-y]
  • Lateral Pressure Change on Phase Transitions of Phosphatidylcholine/Diolein Mixed Membranes.
    T. Kamo, T. Handa, M. Nakano
    Colloid Surf. B: Biointerfaces 104, 128-132 (2013).
    [dx.doi.org/10.1016/j.colsurfb.2012.11.024]

  • Continuous Monitoring of Phospholipid Vesicle Hydrolysis by Phospholipase D (PLD) Reveals Differences in Hydrolysis by PLDs from 2 Streptomyces Species.
    S. Hirano, K. Sekine, T. Handa, M. Nakano
    Colloid Surf. B: Biointerfaces 94, 1-6 (2012).
    [dx.doi.org/10.1016/j.colsurfb.2011.11.059]
  • Effect of Cholesterol on Binding of Amphipathic Helices to Lipid Emulsions.
    M. Ozawa, T. Handa, M. Nakano
    J. Phys. Chem. B 116(1), 476-482 (2012).
    [dx.doi.org/10.1021/jp207062h]